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Restriction enzyme gel
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Order of Lab: 1. restriction enzyme digestion; 2. pouring, loading and running a gel; 3. gel analysis (staining and picture).
Instructional Powerpoint Presentations
Note that the lab protocols here include older versions to show progression of the protocols. If you work on these, be sure to use the most recent version:
Restriction+Enzyme+Lab+revision1.doc
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33 KB
Restriction Enzyme web 2006.mov
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3 MB
Preparing+an+agarose+gel+revised1.doc
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363 KB
Running+a+Gel+revision+1.doc
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44 KB
Loading a gel.ppt
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6 MB
Gel safety.doc
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98 KB
Restriction digest safety.doc
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33 KB
Potential Wrap-Around Stories
fishing story.doc
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120 KB
poodle story.doc
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109 KB
Pearl DNA story.doc
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29 KB
Content flow for background powerpoint
Vocabulary
Lesson Plan Outline
GK-12 Lesson Plan Outline.doc
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56 KB
DNA model activity
2006 DNA LESSON FOR KATS.doc
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154 KB
Restriction Enzyme Powerpoint (by Dustin)
Restriction enzymes.ppt
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639 KB
Restriction Enzyme Student Handout
GK-12 Restriction Enzyme Lab student version - final.doc
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62 KB
Running a Gel Student Handout
GK-12 Preparing DNA Samples and Running a Gel -with Standards - final.doc
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177 KB
DNA Extraction Student Handout
GK-12 DNA extraction by freeze thaw student version - final.doc
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48 KB
PCR PowerPoint
GK-12 PCRSimulation-2.ppt
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7 MB
PCR Pencil/Paper Exercise
GK-12 PCR Student Exercise.html
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10 KB
PCR Lab Activity Student Handout
GK-12 Polymerase Chain Reaction student version - Final.doc
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60 KB
Related Links:
Nearly our protocols, presented very well:
http://www-saps.plantsci.cam.ac.uk/worksheets/scotland/dna.htm
http://bioteach.ubc.ca/TeachingResources/DoingScience/VirtualLabDNA/vlabFrame.html
This is web site for gel electrophoresis preparation:
http://learn.genetics.utah.edu/units/biotech/gel/
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Instructional Powerpoint Presentations
Note that the lab protocols here include older versions to show progression of the protocols. If you work on these, be sure to use the most recent version:
Potential Wrap-Around Stories
Content flow for background powerpoint
Vocabulary
Lesson Plan Outline
DNA model activity
Restriction Enzyme Powerpoint (by Dustin)
Restriction Enzyme Student Handout
Running a Gel Student Handout
DNA Extraction Student Handout
PCR PowerPoint
PCR Pencil/Paper Exercise
PCR Lab Activity Student Handout
Related Links:
Nearly our protocols, presented very well:
http://www-saps.plantsci.cam.ac.uk/worksheets/scotland/dna.htm
http://bioteach.ubc.ca/TeachingResources/DoingScience/VirtualLabDNA/vlabFrame.html
This is web site for gel electrophoresis preparation:
http://learn.genetics.utah.edu/units/biotech/gel/